X-ray structural analysis and biophysical assays in drug discovery

Novel Approaches and Assays in Drug Discovery

High-throughput X-ray techniques and drug discovery.

In the past two decades the promise of structure-based drug design has continued to attract significant interest from the pharmaceutical industry. The initial wave of enthusiasm in the late eighties resulted in some notable successes, for example, the crystal structures of HIV protease and influenza neuraminidase were used to design Viracept and Relenza, both drugs currently used in anti-viral ...

Ethnobotany, Ethnopharmacology and Drug Discovery

     This manuscript is a review of a number of ethnobotany and ethnopharmacology texts and papers in order to arrive at current understanding and scope of ethnobotany and ethnopharmacology. It includes either a brief definition of both fields and presenting some examples on them. Also it has a glimpse on the geographical coverage of research works throughout the world, important issue of prope...

Small-Angle X-ray Scattering Screening Complements Conventional Biophysical Analysis: Comparative Structural and Biophysical Analysis of Monoclonal Antibodies IgG1, IgG2, and IgG4

A crucial step in the development of therapeutic monoclonal antibodies is the selection of robust pharmaceutical candidates and screening of efficacious protein formulations to increase the resistance toward physicochemical degradation and aggregation during processing and storage. Here, we introduce small-angle X-ray scattering (SAXS) to characterize antibody solution behavior, which strongly ...

Structural genomics and drug discovery

Structure determination has already proven useful for lead optimization and direct drug design. The number of high-resolution structures available in public databases today exceeds 30,000 and will definitely aid in structure-based drug design. Structural genomics approaches covering whole genomes, topologically similar proteins or gene families are great assets for further progress in the devel...